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Cloning and characterization of the Escherichia coli gene coding for alkaline phosphatase.
The Escherichia coli structural gene for alkaline phosphatase, phoA, and a promoter-like mutant of phoA, called pho-1003(Bin) phoA+, were cloned by using plasmid vectors. Initially, these genes were cloned on deoxyribonucleic acid fragments of 28.9 kilobases (kb). Subsequently, they were subcloned o...
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| Formato: | Artigo |
| Idioma: | Inglês |
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1981
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| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC217010/ https://ncbi.nlm.nih.gov/pubmed/6260755 |
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