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Transformation in Escherichia coli: stages in the process.

Transformation experiments with Escherichia coli recipient cells and linear chromosomal deoxyribonucleic acid (DNA) are reported. E. coli can be rendered competent for DNA uptake by a temperature shock (0 degrees C leads to 42 degrees C leads to 0 degrees C) of the recipient cells in the presence of...

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Détails bibliographiques
Publié dans:J Bacteriol
Auteurs principaux: Bergmans, H E, van Die, I M, Hoekstra, W P
Format: Artigo
Langue:Inglês
Publié: American Society for Microbiology (ASM) 1981
Sujets:
Accès en ligne:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC216999/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7012133/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.146.2.564-570.1981
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