Strategic Approach To Produce Low-Cost, Efficient, and Stable Competitive Internal Controls for Detection of RNA Viruses by Use of Reverse Transcription-PCR

Molecular diagnostics based on reverse transcription (RT)-PCR are routinely complicated by the lack of stable internal controls, leading to falsely negative results. We describe a strategy to produce a stable competitive internal control (CIC) based on a Qβ phage derivative (recombinant Qβ [rQβ]) be...

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Detalhes bibliográficos
Main Authors: Villanova, Gabriela V., Gardiol, Daniela, Taborda, Miguel A., Reggiardo, Virginia, Tanno, Hugo, Rivadeneira, Emilia D., Perez, Germán R., Giri, Adriana A.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 2007
Assuntos:
Virology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2168486/
https://ncbi.nlm.nih.gov/pubmed/17699653
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.02601-06
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