Isolation of mycoplasma membranes by dicyclohexylcarbodiimide-induced lysis.

A simple procedure was devised to prepared membranes from Mycoplasma gallisepticum cells. The cells were lysed in an isosmotic NaCl solution by dicyclohexylcarbodiimide, which blocks ATPase activity and interferes with the regulation of cell volume. The procedure can be used to isolate membranes of...

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Bibliografiske detaljer

Udgivet i:	J Bacteriol
Principais autores:	Shirvan, M H, Rottem, S, Ne'eman, Z, Bittman, R
Format:	Artigo
Sprog:	Inglês
Udgivet:	American Society for Microbiology (ASM) 1982
Fag:	Research Article
Online adgang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC216504/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7061381/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.149.3.1124-1128.1982
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