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Position of the lacZX90 mutation and hybridization between complete and incomplete beta-galactosidase.

The position of the termination codon in lacZX90 was determined by isolation of a lac+ revertant. Lysine was found to replace tyrosine at position 1,012 of beta-galactosidase, indicating that X90 protein lacked the carboxyl-terminal 10 residues. A heat- and urea-sensitive hybrid enzyme was formed in...

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Detalhes bibliográficos
Publicado no:J Bacteriol
Principais autores: Mandecki, W, Fowler, A V, Zabin, I
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 1981
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC216095/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6790520/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.147.2.694-697.1981
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