Molecular cloning of an ornithine-activating fragment of the gramicidin S synthetase 2 gene from Bacillus brevis and its expression in Escherichia coli.

Partially digested chromosomal DNA of Bacillus brevis ATCC 9999, a producer of the cyclic peptide antibiotic gramicidin S, was ligated into the BamHI site of the Escherichia coli expression vector pUR2-Bam. The ligated molecules were used to transfer E. coli to ampicillin resistance. Of 5 X 10(3) co...

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Détails bibliographiques
Auteurs principaux: Krause, M, Marahiel, M A, von Döhren, H, Kleinkauf, H
Format: Artigo
Langue:Inglês
Publié: 1985
Sujets:
Research Article
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC215892/
https://ncbi.nlm.nih.gov/pubmed/3888956
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