Molecular cloning of an ornithine-activating fragment of the gramicidin S synthetase 2 gene from Bacillus brevis and its expression in Escherichia coli.

Partially digested chromosomal DNA of Bacillus brevis ATCC 9999, a producer of the cyclic peptide antibiotic gramicidin S, was ligated into the BamHI site of the Escherichia coli expression vector pUR2-Bam. The ligated molecules were used to transfer E. coli to ampicillin resistance. Of 5 X 10(3) co...

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Detalhes bibliográficos

Publicado no:	J Bacteriol
Principais autores:	Krause, M, Marahiel, M A, von Döhren, H, Kleinkauf, H
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1985
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC215892/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3888956/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.162.3.1120-1125.1985
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