Method for gene replacement in Pseudomonas aeruginosa used in construction of recA mutants: recA-independent instability of alginate production.

The availability of a technique for site-directed mutagenesis by gene replacement provides a powerful tool for genetic analysis in any bacterial species. We report here a general technique for gene replacement in Pseudomonas aeruginosa. Genes on fragments of cloned P. aeruginosa DNA, altered by tran...

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Detalhes bibliográficos

Publicado no:	J Bacteriol
Principais autores:	Ohman, D E, West, M A, Flynn, J L, Goldberg, J B
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1985
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC215884/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2987182/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.162.3.1068-1074.1985
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