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Isolation and expression of the Bradyrhizobium japonicum adenylate cyclase gene (cya) in Escherichia coli.

A 5.0-kilobase-pair HindIII fragment of Bradyrhizobium japonicum DNA containing the cya gene which encodes adenylate cyclase was isolated as an insert in pBR322, using marker rescue of the maltose-negative phenotype of an Escherichia coli cya mutant for identification. The isolated B. japonicum DNA...

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Main Authors: Guerinot, M L, Chelm, B K
格式: Artigo
語言:Inglês
出版: 1984
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC215772/
https://ncbi.nlm.nih.gov/pubmed/6090388
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