Molecular cloning of pectate lyase genes from Erwinia chrysanthemi and their expression in Escherichia coli.
A genomic library of Erwinia chrysanthemi EC16 was constructed in plasmid pHC79, and seven putative pectate lyase (PL) clones in Escherichia coli were selected on pectate agar. Six of the recombinant cosmids contained a common PstI fragment of ca. 8.2 kilobases (kb). Subcloning of this fragment in e...
Guardat en:
| Publicat a: | J Bacteriol |
|---|---|
| Autors principals: | , , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
American Society for Microbiology (ASM)
1984
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC215732/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6090392/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.159.3.825-831.1984 |
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