In Situ Bipolar Electroporation for Localized Cell Loading with Reporter Dyes and Investigating Gap Junctional Coupling

Electroporation is generally used to transfect cells in suspension, but the technique can also be applied to load a defined zone of adherent cells with substances that normally do not permeate the plasma membrane. In this case a pulsed high-frequency oscillating electric field is applied over a smal...

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Autores principales: De Vuyst, Elke, De Bock, Marijke, Decrock, Elke, Van Moorhem, Marijke, Naus, Christian, Mabilde, Cyriel, Leybaert, Luc
Formato: Artigo
Lenguaje:Inglês
Publicado: The Biophysical Society 2008
Materias:
Channels, Receptors, and Electrical Signaling
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC2157259/
https://ncbi.nlm.nih.gov/pubmed/17872956
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.107.109470
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