Cloning and expression of a gene segment encoding the enzymatic moiety of Pseudomonas aeruginosa exotoxin A.

Using the broad-host-range plasmid vector pRO1614, we cloned a segment of the gene from Pseudomonas aeruginosa PA103 encoding the enzymatically active part of the exotoxin A protein. Expression of the cloned gene segment has been achieved both in Escherichia coli and in a nontoxigenic P. aeruginosa...

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Détails bibliographiques

Publié dans:	J Bacteriol
Auteurs principaux:	Mozola, M A, Wilson, R B, Jordan, E M, Draper, R K, Clowes, R C
Format:	Artigo
Langue:	Inglês
Publié:	American Society for Microbiology (ASM) 1984
Sujets:	Research Article
Accès en ligne:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC215698/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6086583/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.159.2.683-687.1984
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