Purification and characterization of a dUTPase from Acholeplasma laidlawii B-PG9.
dUTP was purified 120-fold from extracts of Acholeplasma laidlawii B-PG9 by Blue-Sepharose, Phenyl-Sepharose, hydroxyapatite, and DEAE-Sephacel chromatography techniques. The only substrate for the enzyme was dUTP with an apparent Km of 4.5 microM. The only reaction products were dUMP and PPi. The d...
Na minha lista:
| Publicado no: | J Bacteriol |
|---|---|
| Principais autores: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Society for Microbiology (ASM)
1984
|
| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC215625/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6145699/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.159.1.278-282.1984 |
| Tags: |
Sem tags, seja o primeiro a adicionar uma tag!
|