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Use of a cloned DNA fragment to analyze the fate of donor DNA in transformation of Streptococcus pneumoniae.

The integration of donor label into the recipient fragment is followed during transformation of Streptococcus pneumoniae. The method used involves gel analysis of restriction endonuclease-treated recipient DNA after recombination with a radioactively labeled homologous cloned fragment.

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Bibliographic Details
Published in:J Bacteriol
Main Authors: Méjean, V, Claverys, J P
Format: Artigo
Language:Inglês
Published: American Society for Microbiology (ASM) 1984
Subjects:
Online Access:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC215570/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6327632/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.158.3.1175-1178.1984
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