Cloning and characterization of the Bacillus licheniformis gene coding for alkaline phosphatase.
The structural gene for alkaline phosphatase (orthophosphoric monoester phosphohydrolase; EC 3.1.3.1) of Bacillus licheniformis MC14 was cloned into the Pst1 site of pMK2004 from chromosomal DNA. The gene was cloned on an 8.5-kilobase DNA fragment. A restriction map was developed, and the gene was s...
Guardat en:
| Publicat a: | J Bacteriol |
|---|---|
| Autor principal: | |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
American Society for Microbiology (ASM)
1984
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC215538/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6327655/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.158.3.978-982.1984 |
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