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Construction of a single-copy promoter vector and its use in analysis of regulation of the transposon Tn10 tetracycline resistance determinant.

The construction and characterization of a promoter expression vector, lambda RS205 , is described. lambda RS205 can be used for the in vitro construction of transcriptional (operon) fusions to the lacZ gene of Escherichia coli K-12. The level of beta-galactosidase activity in lysogens of lambda RS2...

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Autors principals: Bertrand, K P, Postle, K, Wray, L V, Reznikoff, W S
Format: Artigo
Idioma:Inglês
Publicat: 1984
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC215528/
https://ncbi.nlm.nih.gov/pubmed/6327648
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