Construction of a single-copy promoter vector and its use in analysis of regulation of the transposon Tn10 tetracycline resistance determinant.

The construction and characterization of a promoter expression vector, lambda RS205 , is described. lambda RS205 can be used for the in vitro construction of transcriptional (operon) fusions to the lacZ gene of Escherichia coli K-12. The level of beta-galactosidase activity in lysogens of lambda RS2...

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Detalhes bibliográficos

Publicado no:	J Bacteriol
Principais autores:	Bertrand, K P, Postle, K, Wray, L V, Reznikoff, W S
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1984
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC215528/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6327648/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.158.3.910-919.1984
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