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Transfer of recombinant plasmids containing the gene for DpnII DNA methylase into strains of Streptococcus pneumoniae that produce DpnI or DpnII restriction endonucleases.

Plasmid transfer via the transformation pathway of Streptococcus pneumoniae was weakly restricted by the DpnI or DpnII restriction endonuclease, either of which gave a reduction only to 0.4, compared with phage infection, which was restricted to 10(-5). The greater sensitivity of plasmid transfer co...

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Détails bibliographiques
Publié dans:J Bacteriol
Auteurs principaux: Lacks, S A, Springhorn, S S
Format: Artigo
Langue:Inglês
Publié: American Society for Microbiology (ASM) 1984
Sujets:
Accès en ligne:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC215527/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6327647/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.158.3.905-909.1984
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