Method for determining whether a gene of Escherichia coli is essential: application to the polA gene.

We have developed a general method for determining whether a gene of Escherichia coli is essential for viability. The method requires cloned DNA spanning the gene in question and a reasonably detailed genetic and physical map of the cloned segment. Using this information, one constructs a deletion o...

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Bibliographic Details
Main Authors: Joyce, C M, Grindley, N D
Format: Artigo
Language:Inglês
Published: 1984
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC215477/
https://ncbi.nlm.nih.gov/pubmed/6233260
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