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Plasmid insertion mutagenesis and lac gene fusion with mini-mu bacteriophage transposons.

Small bacteriophage Mu transposable elements containing the lac operon structural genes were constructed to facilitate the isolation and use of Mu insertions and lac gene fusions. These mini-Mu elements have selectable genes for either ampicillin or kanamycin resistance and can be used to form both...

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Bibliographic Details
Main Authors: Castilho, B A, Olfson, P, Casadaban, M J
Format: Artigo
Language:Inglês
Published: 1984
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC215454/
https://ncbi.nlm.nih.gov/pubmed/6327606
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