Replacement of the Bacillus subtilis subtilisin structural gene with an In vitro-derived deletion mutation.
The entire subtilisin structural gene from Bacillus subtilis I168 has been cloned, and its nucleotide sequence has been determined. When expressed on a high-copy-number shuttle vector, a fivefold increase in serine protease activity was observed. The DNA sequence of the gene is 80% homologous to the...
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| Publicado no: | J Bacteriol |
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| Principais autores: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Society for Microbiology (ASM)
1984
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC215443/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6427178/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.158.2.411-418.1984 |
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