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Replacement of the Bacillus subtilis subtilisin structural gene with an In vitro-derived deletion mutation.

The entire subtilisin structural gene from Bacillus subtilis I168 has been cloned, and its nucleotide sequence has been determined. When expressed on a high-copy-number shuttle vector, a fivefold increase in serine protease activity was observed. The DNA sequence of the gene is 80% homologous to the...

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Autors principals: Stahl, M L, Ferrari, E
Format: Artigo
Idioma:Inglês
Publicat: 1984
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC215443/
https://ncbi.nlm.nih.gov/pubmed/6427178
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