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Expression, purification, crystallization and preliminary X-ray analysis of the human RuvB-like protein RuvBL1

RuvBL1, an evolutionary highly conserved protein related to the AAA(+) family of ATPases, has been crystallized using the hanging-drop vapour-diffusion method at 293 K. The crystals are hexagonal and belong to space group P6, with unit-cell parameters a = b = 207.1, c = 60.7 Å and three molecules in...

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Detalhes bibliográficos
Main Authors: Gorynia, Sabine, Matias, Pedro M., Gonçalves, Susana, Coelho, Ricardo, Lopes, Gonçalo, Thomaz, Mónica, Huber, Martina, Haendler, Bernard, Donner, Peter, Carrondo, Maria Arménia
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2005
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2150925/
https://ncbi.nlm.nih.gov/pubmed/16511264
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309105041400
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