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In situ assay for 5-aminolevulinate dehydratase and application to the study of a catabolite repression-resistant Saccharomyces cerevisiae mutant.

To facilitate the study of the effects of carbon catabolite repression and mutations on 5-aminolevulinate dehydratase (EC 4.2.1.24) from Saccharomyces cerevisiae, a sensitive in situ assay was developed, using cells permeabilized by five cycles of freezing and thawing. Enzymatic activity was measure...

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Detalhes bibliográficos
Publicado no:J Bacteriol
Principais autores: Borralho, L M, Panek, A D, Malamud, D R, Sanders, H K, Mattoon, J R
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 1983
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC215062/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6352674/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.156.1.141-147.1983
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