Three-Dimensional Visualization of Transcription Sites and Their Association with Splicing Factor–Rich Nuclear Speckles

Transcription sites are detected by labeling nascent transcripts with BrUTP in permeabilized 3T3 mouse fibroblasts followed by laser scanning confocal microscopy. Inhibition and enzyme digestion studies confirm that the labeled sites are from RNA transcripts and that RNA polymerase I (RP I) and II (...

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Main Authors: Wei, Xiangyun, Somanathan, Suryanarayan, Samarabandu, Jagath, Berezney, Ronald
Format: Artigo
Jezik:Inglês
Izdano: The Rockefeller University Press 1999
Teme:
Original Article
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC2150559/
https://ncbi.nlm.nih.gov/pubmed/10444064
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