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Rapid method for isolation and screening of cytochrome c oxidase-deficient mutants of Saccharomyces cerevisiae.

We describe here a new method for the specific isolation of cytochrome c oxidase-deficient mutants of Saccharomyces cerevisiae. One unique feature of the method is the use of tetramethyl-p-phenylenediamine as a cytochrome c oxidase activity stain for yeast colonies. The staining of yeast colonies by...

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Détails bibliographiques
Publié dans:J Bacteriol
Auteurs principaux: McEwen, J E, Cameron, V L, Poyton, R O
Format: Artigo
Langue:Inglês
Publié: American Society for Microbiology (ASM) 1985
Sujets:
Accès en ligne:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC214973/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2982789/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.161.3.831-835.1985
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