Efficient transformation of Erwinia carotovora subsp. carotovora and E. carotovora subsp. atroseptica.

We used a modified version of the method of Hanahan (D. Hanahan, J. Mol. Biol. 166:557-580, 1983) to transform Erwinia carotovora subsp. carotovora and E. carotovora subsp. atroseptica with the plasmids pBR322, pBR325, and pAT153. The transformation frequency ranged from 1 X 10(2) to 4 X 10(4) colon...

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Detalles Bibliográficos

Publicado en:	J Bacteriol
Principais autores:	Hinton, J C, Perombelon, M C, Salmond, G P
Formato:	Artigo
Idioma:	Inglês
Publicado:	American Society for Microbiology (ASM) 1985
Assuntos:	Research Article
Acceso en liña:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC214956/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3968041/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.161.2.786-788.1985
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