Molecular cloning of the phenylalanine ammonia lyase gene from Rhodosporidium toruloides in Escherichia coli K-12.

A genomic library of Rhodosporidium toruloides DNA was constructed in bacteriophage lambda 1059. Recombinant phage containing phenylalanine ammonia lyase (PAL) gene sequences were identified by using 32P-labeled cDNA to partially purified PAL mRNA. The PAL gene was subcloned on an 8.5-kilobase PstI...

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Detalhes bibliográficos

Publicado no:	J Bacteriol
Principais autores:	Gilbert, H J, Clarke, I N, Gibson, R K, Stephenson, J R, Tully, M
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1985
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC214873/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2981805/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.161.1.314-320.1985
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