Highly efficient protoplast transformation system for Streptococcus faecalis and a new Escherichia coli-S. faecalis shuttle vector.

A highly efficient protoplast transformation system for Streptococcus faecalis has been developed by systematically optimizing different parameters. Up to 10(6) transformants per micrograms of DNA were consistently obtained within 3 days, and cell wall regeneration of protoplasts was virtually 100%....

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Autors principals: Wirth, R, An, F Y, Clewell, D B
Format: Artigo
Idioma:Inglês
Publicat: 1986
Matèries:
Research Article
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC214503/
https://ncbi.nlm.nih.gov/pubmed/3005240
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