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Mutations of fumarase that distinguish between the active site and a nearby dicarboxylic acid binding site.

Two mutant forms of fumarase C from E. coli have been made using PCR and recombinant DNA. The recombinant form of the protein included a histidine arm on the C-terminal facilitating purification. Based on earlier studies, two different carboxylic acid binding sites, labeled A- and B-, were observed...

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Библиографические подробности
Главные авторы: Weaver, T., Lees, M., Banaszak, L.
Формат: Artigo
Язык:Inglês
Опубликовано: Cold Spring Harbor Laboratory Press 1997
Предметы:
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC2144750/
https://ncbi.nlm.nih.gov/pubmed/9098893
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