Human RhoA/RhoGDI complex expressed in yeast: GTP exchange is sufficient for translocation of RhoA to liposomes.

The human small GTPase, RhoA, expressed in Saccharomyces cerevisiae is post-translationally processed and, when co-expressed with its cytosolic inhibitory protein, RhoGDI, spontaneously forms a heterodimer in vivo. The RhoA/RhoGDI complex, purified to greater than 98% at high yield from the yeast cy...

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Detalhes bibliográficos
Main Authors: Read, P. W., Liu, X., Longenecker, K., Dipierro, C. G., Walker, L. A., Somlyo, A. V., Somlyo, A. P., Nakamoto, R. K.
Formato: Artigo
Idioma:Inglês
Publicado em: Cold Spring Harbor Laboratory Press 2000
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2144558/
https://ncbi.nlm.nih.gov/pubmed/10716190
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