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Analysis of the extent of unfolding of denatured insulin-like growth factor.
Insulin-like growth factor (IGF-1) contains three disulfide bonds. In the presence of denaturant and thiol catalyst, IGF-1 shuffles its native disulfide bonds and denatures to form a mixture of scrambled isomers. The composition of scrambled IGF varies under different denaturing conditions. Among th...
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Main Authors: | , , |
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Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
Cold Spring Harbor Laboratory Press
1999
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Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2144389/ https://ncbi.nlm.nih.gov/pubmed/10422834 |
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