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The Mycobacterium tuberculosis recA intein can be used in an ORFTRAP to select for open reading frames.

The DNA repair protein RecA of Mycobacterium tuberculosis contains an intein, a self-splicing protein element. We have employed this Mtu recA intein to create a selection system for successful intein splicing by inserting it into a kanamycin-resistance gene so that functional antibiotic resistance c...

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Bibliografische gegevens
Hoofdauteurs: Daugelat, S., Jacobs, W. R.
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: Cold Spring Harbor Laboratory Press 1999
Onderwerpen:
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2144272/
https://ncbi.nlm.nih.gov/pubmed/10091667
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