Cloning, overproduction, and purification of the B2 subunit of ribonucleoside-diphosphate reductase.
The nrdB gene, which encodes the B2 subunit of Escherichia coli ribonucleotide reductase (EC 1.17.4.1), was cloned into multicopy plasmid pSPS2. This vector, which contains the pL promoter of bacteriophage lambda and the tetracycline resistance gene of pBR322, was transformed into a lysogenic host w...
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| Udgivet i: | J Bacteriol |
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| Principais autores: | , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
American Society for Microbiology (ASM)
1986
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC214425/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3511029/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.165.2.363-366.1986 |
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