An internal affinity-tag for purification and crystallization of the siderophore receptor FhuA, integral outer membrane protein from Escherichia coli K-12.

FhuA (Mr 78,992, 714 amino acids), siderophore receptor for ferrichrome-iron in the outer membrane of Escherichia coli, was affinity tagged, rapidly purified, and crystallized. To obtain FhuA in quantities sufficient for crystallization, a hexahistidine tag was genetically inserted into the fhuA gen...

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Détails bibliographiques
Auteurs principaux: Ferguson, A. D., Breed, J., Diederichs, K., Welte, W., Coulton, J. W.
Format: Artigo
Langue:Inglês
Publié: Cold Spring Harbor Laboratory Press 1998
Sujets:
Research Article
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC2144053/
https://ncbi.nlm.nih.gov/pubmed/9684898
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