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Exploring sequence constraints on an interhelical turn using in vivo selection for catalytic activity.

The role of interhelical turns in determining protein structure has been investigated previously in relatively simple four-helix-bundle proteins using combinatorial mutagenesis coupled with screening for functional variants. To assess the tolerance to sequence substitution of a short, interhelical t...

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Detalles Bibliográficos
Main Authors: MacBeath, G., Kast, P., Hilvert, D.
Formato: Artigo
Idioma:Inglês
Publicado: Cold Spring Harbor Laboratory Press 1998
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC2143915/
https://ncbi.nlm.nih.gov/pubmed/9521108
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