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Determination of pKa values of the histidine side chains of phosphatidylinositol-specific phospholipase C from Bacillus cereus by NMR spectroscopy and site-directed mutagenesis.

Two active site histidine residues have been implicated in the catalysis of phosphatidylinositol-specific phospholipase C (PI-PLC). In this report, we present the first study of the pKa values of histidines of a PI-PLC. All six histidines of Bacillus cereus PI-PLC were studied by 2D NMR spectroscopy...

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Bibliografske podrobnosti
Main Authors: Liu, T., Ryan, M., Dahlquist, F. W., Griffith, O. H.
Format: Artigo
Jezik:Inglês
Izdano: Cold Spring Harbor Laboratory Press 1997
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC2143799/
https://ncbi.nlm.nih.gov/pubmed/9300493
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