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Increased production of low molecular weight recombinant proteins in Escherichia coli.

A general method for obtaining high-level production of low molecular weight proteins in Escherichia coli is described. This method is based on the use of a novel Met-Xaa-protein construction which is formed by insertion of a single amino acid residue (preferably Arginine or Lysine) between the N-te...

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Autori principali: Belagaje, R. M., Reams, S. G., Ly, S. C., Prouty, W. F.
Natura: Artigo
Lingua:Inglês
Pubblicazione: Cold Spring Harbor Laboratory Press 1997
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC2143793/
https://ncbi.nlm.nih.gov/pubmed/9300495
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