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Design of an expression system for detecting folded protein domains and mapping macromolecular interactions by NMR.

Two protein expression vectors have been designed for the preparation of NMR samples. The vectors encode the immunoglobulin-binding domain of streptococcal protein G (GB1 domain) linked to the N-terminus of the desired proteins. This fusion strategy takes advantage of the small size, stable fold, an...

Täydet tiedot

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Bibliografiset tiedot
Päätekijät: Huth, J. R., Bewley, C. A., Jackson, B. M., Hinnebusch, A. G., Clore, G. M., Gronenborn, A. M.
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: Cold Spring Harbor Laboratory Press 1997
Aiheet:
Linkit:https://ncbi.nlm.nih.gov/pmc/articles/PMC2143577/
https://ncbi.nlm.nih.gov/pubmed/9385638
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