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Limited proteolysis of native proteins: the interaction between avidin and proteinase K.

Avidin is a tetramer of 16-kDa subunits that have a high affinity for biotin. Proteolysis of native apoavidin by proteinase K results in a limited attack at the loop between beta-strands 3 and 4, involving amino acids 38-43. Specifically, sites of proteolysis are at Thr 40-Ser 41 and Asn 42-Glu 43....

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Autors principals: Ellison, D., Hinton, J., Hubbard, S. J., Beynon, R. J.
Format: Artigo
Idioma:Inglês
Publicat: Cold Spring Harbor Laboratory Press 1995
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2143173/
https://ncbi.nlm.nih.gov/pubmed/7670376
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