Purification, characterization, and immunological properties of fumarase from Euglena gracilis var. bacillaris.
A rapid three-step procedure utilizing heat treatment, ammonium sulfate fractionation, and affinity chromatography on Matrex gel Orange A purified fumarase (EC 4.2.1.2) 632-fold with an 18% yield from crude extracts of Euglena gracilis var. bacillaris. The apparent molecular weight of the native enz...
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| Udgivet i: | J Bacteriol |
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| Principais autores: | , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
American Society for Microbiology (ASM)
1985
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC214317/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3932328/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.164.2.762-768.1985 |
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