How to measure and predict the molar absorption coefficient of a protein.

The molar absorption coefficient, epsilon, of a protein is usually based on concentrations measured by dry weight, nitrogen, or amino acid analysis. The studies reported here suggest that the Edelhoch method is the best method for measuring epsilon for a protein. (This method is described by Gill an...

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Bibliographic Details
Main Authors: Pace, C. N., Vajdos, F., Fee, L., Grimsley, G., Gray, T.
Format: Artigo
Language:Inglês
Published: Cold Spring Harbor Laboratory Press 1995
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2143013/
https://ncbi.nlm.nih.gov/pubmed/8563639
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