Properties of a recombinant human hemoglobin with aspartic acid 99(beta), an important intersubunit contact site, substituted by lysine.

Site-directed mutagenesis of an important subunit contact site, Asp-99(beta), by a Lys residue (D99K(beta)) was proven by sequencing the entire beta-globin gene and the mutant tryptic peptide. Oxygen equilibrium curves of the mutant hemoglobin (Hb) (2-15 mM in heme) indicated that it had an increase...

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Détails bibliographiques
Auteurs principaux: Yanase, H., Cahill, S., Martin de Llano, J. J., Manning, L. R., Schneider, K., Chait, B. T., Vandegriff, K. D., Winslow, R. M., Manning, J. M.
Format: Artigo
Langue:Inglês
Publié: Cold Spring Harbor Laboratory Press 1994
Sujets:
Research Article
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC2142911/
https://ncbi.nlm.nih.gov/pubmed/7987216
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