Expression, purification, and characterization of thymidylate synthase from Lactococcus lactis.

The thymidylate synthase (TS) gene from Lactococcus lactis has been highly expressed in Escherichia coli. The TS protein was purified by sequential chromatography on Q-Sepharose and phenyl-Sepharose. Six grams of cell pellet yielded 140 mg of homogeneous TS. TS is a highly conserved enzyme, and seve...

Полное описание

Сохранить в:
Библиографические подробности
Главные авторы: Greene, P. J., Yu, P. L., Zhao, J., Schiffer, C. A., Santi, D.
Формат: Artigo
Язык:Inglês
Опубликовано: Cold Spring Harbor Laboratory Press 1994
Предметы:
Research Article
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC2142890/
https://ncbi.nlm.nih.gov/pubmed/7920258
Метки: Добавить метку
Нет меток, Требуется 1-ая метка записи!

Схожие документы