Cloning and expression in Escherichia coli of a gene coding for a chondroitin lyase from Bacteroides thetaiotaomicron.

We cloned the gene for one of the two chondroitin lyases of Bacteroides thetaiotaomicron into the cosmid vector pHC79 and subcloned it into pBR328. No proteins the size of B. thetaiotaomicron chondroitin lyase I or II (104 to 108 kilodaltons) were detectable in maxicell or in vitro transcription-tra...

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Detalhes bibliográficos

Publicado no:	J Bacteriol
Principais autores:	Guthrie, E P, Shoemaker, N B, Salyers, A A
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1985
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC214281/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2997114/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.164.2.510-515.1985
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