High-sensitivity sequencing of large proteins: partial structure of the rapamycin-FKBP12 target.

We report on studies leading to refinements of various steps of the protein internal sequencing process. Specifically, the developments comprise (1) higher-sensitivity chemical sequencing through background reduction; (2) improved peptide recovery from rapid in situ digests of nanogram amount, nitro...

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Détails bibliographiques
Auteurs principaux: Erdjument-Bromage, H., Lui, M., Sabatini, D. M., Snyder, S. H., Tempst, P.
Format: Artigo
Langue:Inglês
Publié: Cold Spring Harbor Laboratory Press 1994
Sujets:
Research Article
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC2142775/
https://ncbi.nlm.nih.gov/pubmed/7756997
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