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Polyethylene glycol-facilitated transformation of Bacteroides fragilis with plasmid DNA.

A method for the transformation of Bacteroides fragilis with plasmid DNA was developed by using the clindamycin resistance plasmid pBFTM10 as the source of transforming DNA. The method was technically simple to perform and resulted in an average of 4.2 X 10(3) transformants per microgram of pBFTM10...

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Detalhes bibliográficos
Publicado no:J Bacteriol
Autor principal: Smith, C J
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 1985
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC214267/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/4044530/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.164.1.466-469.1985
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