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Novel one-step cloning vector with a transposable element: application to the Myxococcus xanthus genome.

A new strategy was developed for rapid cloning of genes with a transposon mutation library. We constructed a transposon designated TnV that was derived from Tn5 and consists of the gene coding for neomycin phosphotransferase II as well as the replication origin of an Escherichia coli plasmid, pSC101...

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Autors principals: Furuichi, T, Inouye, M, Inouye, S
Format: Artigo
Idioma:Inglês
Publicat: 1985
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC214239/
https://ncbi.nlm.nih.gov/pubmed/2995310
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