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Bacterial expression and photoaffinity labeling of a pheromone binding protein.

The first high-level production of a binding-active odorant binding protein is described. The expression cassette polymerase chain reaction was used to generate a DNA fragment encoding the pheromone binding protein (PBP) of the male moth Antheraea polyphemus. Transformation of Escherichia coli cells...

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Autor principal: Prestwich, G. D.
Format: Artigo
Idioma:Inglês
Publicat: Cold Spring Harbor Laboratory Press 1993
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2142376/
https://ncbi.nlm.nih.gov/pubmed/8453379
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