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Membrane particles from Escherichia coli and Bacillus subtilis, containing penicillin-binding proteins and enriched for chromosomal-origin DNA.

Rapid-sedimenting DNA-membrane complexes were obtained from both Bacillus subtilis and Escherichia coli by a method involving gentle lysis followed by restriction enzyme digestion and sucrose gradient fractionation. These complexes were substantially enriched in chromosomal origin DNA, and in B. sub...

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Detalhes bibliográficos
Main Authors: Bone, E J, Todd, J A, Ellar, D J, Sargent, M G, Wyke, A W
Formato: Artigo
Idioma:Inglês
Publicado em: 1985
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC214229/
https://ncbi.nlm.nih.gov/pubmed/3930465
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