Isolation and characterization of porcine somatotropin containing a succinimide residue in place of aspartate129.

Aspartate129 in porcine somatotropin was converted into a cyclic imide residue (succinimide) under acidic solution conditions. Reversed-phase high performance liquid chromatography was utilized to isolate and quantitate this altered species, which accounted for approximately 30% of the total protein...

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Main Authors: Violand, B. N., Schlittler, M. R., Kolodziej, E. W., Toren, P. C., Cabonce, M. A., Siegel, N. R., Duffin, K. L., Zobel, J. F., Smith, C. E., Tou, J. S.
Format: Artigo
Sprog:Inglês
Udgivet: Cold Spring Harbor Laboratory Press 1992
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2142126/
https://ncbi.nlm.nih.gov/pubmed/1363933
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