Requirement of a heat-labile factor(s) for in vitro expression of the amp gene of pBR322.
The amp gene of pBR328 and pBR322 was expressed less efficiently at 45 than at 30 degrees C in an in vitro coupled transcription-translation system of Escherichia coli. Preincubation of S30 extract at 45 degrees C reduced specifically its ability to express the amp gene at 30 degrees C, indicating i...
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| Publicado no: | J Bacteriol |
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| Autor principal: | |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Society for Microbiology (ASM)
1987
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC214187/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3316194/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.169.12.5856-5858.1987 |
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