Facile autoplast generation and transformation in Bacillus thuringiensis subsp. kurstaki.

We describe a method for maximizing the rate of conversion of Bacillus thuringiensis subsp. kurstaki vegetative cells to osmotically fragile forms in the absence of exogenously added enzymes. Optimal generation of autoplasts occurred in 50 mM sodium acetate buffer (pH 7.0) at 37 degrees C with 10% (...

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Bibliografiske detaljer

Udgivet i:	J Bacteriol
Principais autores:	Crawford, I T, Greis, K D, Parks, L, Streips, U N
Format:	Artigo
Sprog:	Inglês
Udgivet:	American Society for Microbiology (ASM) 1987
Fag:	Research Article
Online adgang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC213967/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3680166/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.169.12.5423-5428.1987
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