Facile autoplast generation and transformation in Bacillus thuringiensis subsp. kurstaki.
We describe a method for maximizing the rate of conversion of Bacillus thuringiensis subsp. kurstaki vegetative cells to osmotically fragile forms in the absence of exogenously added enzymes. Optimal generation of autoplasts occurred in 50 mM sodium acetate buffer (pH 7.0) at 37 degrees C with 10% (...
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| Опубликовано в:: | J Bacteriol |
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| Главные авторы: | , , , |
| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
American Society for Microbiology (ASM)
1987
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC213967/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3680166/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.169.12.5423-5428.1987 |
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